Confirmation of the use of Latex IgM on cerebrospinal fluid for improving stage determination of Human African Trypanosomiasis
نویسندگان
چکیده
Institut de Recherche pour le Développement, UR177 « Trypanosomoses Africaines », International Campus of Baillarguet, IRD / CIRAD, TA 207 / G 34 398 Montpellier cedex 5 France. Institut de Recherche pour le Développement, Génétique et Evolution des Maladies Infectieuses, UMR 2724 IRDCNRS, Equipe "Evolution des Systèmes Symbiotiques", 911 Avenue Agropolis B.P. 64501 34394 Montpellier Cedex 5 France.
منابع مشابه
Stage determination and therapeutic decision in human African trypanosomiasis: value of polymerase chain reaction and immunoglobulin M quantification on the cerebrospinal fluid of sleeping sickness patients in Côte d'Ivoire.
In human African trypanosomiasis (HAT), two disease stages are defined: the first, or haemo-lymphatic stage, and the second, or meningo-encephalitic stage. Stage determination forms the basis of therapeutic decision and is of prime importance, as the drug used to cure second-stage patients has considerable side-effects. However, the tests currently used for stage determination have low sensitiv...
متن کاملHuman African trypanosomiasis: a latex agglutination field test for quantifying IgM in cerebrospinal fluid.
LATEX/IgM, a rapid agglutination test for the semi-quantitative detection of IgM in cerebrospinal fluid of patients with African trypanosomiasis, is described in this article. The lyophilized reagent has been designed for field use and remains stable at 45 degrees C for one year. The test has been evaluated on cerebrospinal fluid samples from trypanosome-infected and non-infected patients, by c...
متن کاملIgM quantification in the cerebrospinal fluid of sleeping sickness patients by a latex card agglutination test.
An increased IgM concentration in cerebrospinal fluid (CSF), occurring as a consequence of massive intrathecal IgM synthesis, is a marker of interest for diagnosis of the meningo-encephalitic stage in human African trypanosomiasis. However, in current practice, IgM in CSF is not determined because of the lack of a simple and robust test that is applicable in African rural regions where the dise...
متن کاملOptions for field diagnosis of human african trypanosomiasis.
Human African trypanosomiasis (HAT) due to Trypanosoma brucei gambiense or T. b. rhodesiense remains highly prevalent in several rural areas of sub-Saharan Africa and is lethal if left untreated. Therefore, accurate tools are absolutely required for field diagnosis. For T. b. gambiense HAT, highly sensitive tests are available for serological screening but the sensitivity of parasitological con...
متن کاملTreatment failure related to intrathecal immunoglobulin M (IgM) synthesis, cerebrospinal fluid IgM, and interleukin-10 in patients with hemolymphatic-stage sleeping sickness.
Human African trypanosomiasis treatment is stage dependent, but the tests used for staging are controversial. Central nervous system involvement and its relationship with suramin treatment failure were assessed in 60 patients with parasitologically confirmed hemolymphatic-stage Trypanosoma brucei gambiense infection (white blood cell count of <or=5/microl and no trypanosomes in the cerebrospina...
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